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Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Biotinylated (B-1105-2)

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Description

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin. Biotinylated GSL I has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs
Recommended UsageFor most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting SugarInhibiting/Eluting Sugar: Mixture of 200 mM galactose (S-9003)/200 mM N-acetylgalactosamine (S-9001).
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificityGalactose, N-Acetylgalactosamine

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine

Applicable patents and legal notices are available at legal notices.

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