This lectin consists of two subunits of 60 kDa which can be dissociated by reducing agents into closely related chains between 27 kDa and 33 kDa. One of the chains appears to be common to the “B” chain of another castor bean lectin, ricin, while the other chain is unique to RCA I. The B chain binds to galactose or N-acetylgalactosamine residues of membrane glycoconjugates.
Biotinylated RCA I has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
Unit Size | 1 mg |
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Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs |
Recommended Usage | For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer. |
Recommended Storage | 2-8 °C; DO NOT FREEZE |
Inhibiting and/or Eluting Sugar | Inhibiting/Eluting Sugar: 200 mM galactose (S-9003) or lactose (S-9004). |
Solution | 10 mM sodium phosphate, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 5 mM lactose |
Concentration | 5 mg active conjugate/ml |
Conjugate | Biotinylated |
Sugar Specificity | Galactose, Lactose |
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting/Eluting Sugar: 200 mM galactose or lactose
Applicable patents and legal notices are available at legal notices.
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