Biotin-dPEG®7-azide, product number QBD-10825, is a water-soluble biotinylation product functionalized with azide for click chemistry use on a short (27 atoms, 30.7 Å) single molecular weight, discrete PEG (dPEG®) chain. This product enables the biotinylation of molecules alkyne-containing molecules and surfaces using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Moreover, the hydrophilic discrete PEG spacer makes biotin highly water soluble, reducing or eliminating aggregation and precipitation of biotin-labeled biomolecules triggered by hydrophobic interactions. Consequently, these products allow new types of biotinylated constructs.
Because Biotin-dPEG®7-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®7-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.
As a biotinylation reagent, Biotin-dPEG®7-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®7-azide.
Unit Size | 100mg, 1000mg |
---|---|
Molecular Weight | 620.32; single compound |
Chemical formula | C₂₆H₄₈N₆O₉S |
CAS | 1334172-75-6 |
Purity | > 98% |
Spacers | dPEG® Spacer is 27 atoms and 30.7 Å |
Shipping | Ambient |
Typical solubility properties (for additional information contact Customer Support) | Methylene chloride, DMAC or DMSO. |
Storage and handling | -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure. |
Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), and specifically see pp. 722-724.
Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
Surface Functionalization Using Catalyst-Free Azide-Alkyne Cycloaddition. Alexander Kuzmin, Andrei Poloukhtine, Margreet A. Wolfert, and Vladimir V. Popik. Bioconjugate Chem. 2010, 21 (11) pp 2076–2085. October 21, 2010. DOI: 10.1021/bc100306u.
Palmitoylation of superoxide dismutase 1(SOD1) is increased for familial ALS-linked SOD1 mutants. Sarah E. Antinone, Ghanashyam D. Ghadge, TuKiet T. Lam, Lijun Wang, Raymond P. Roos and William N. Green. J. Biol. Chem. 2013, 288 (49) pp 1-25. June 12, 2013. DOI: 10.1074/jbc.M113.487231.
Herpes Simplex Virus 2 Infection Impacts Stress Granule Accumulation. Renée L. Finnen, Kyle R. Pangka, and Bruce W. Banfield. J. Virol. 2012, 86 (15) pp 8119–8130. August 1, 2012. doi:10.1128/JVI.00313-12.
Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens. Shixian Lin, Zhenrun Zhang, Hao Xu, Lin Li, She Chen, Jie Li, Ziyang Hao, and Peng R. Chen. J. Am. Chem. Soc. 2011, 133 (50), pp 20581–20587. November 15, 2011. DOI: 10.1021/ja209008w.
Polymer Therapeutics with a Coiled Coil Motif Targeted against Murine BCL1 Leukemia. Robert Pola, Richard Laga, Karel Ulbrich, Irena Sieglová, Vlastimil Král, Milan Fábry, Martina Kabešová, Marek Kovář, and Michal Pechar. Biomacromolecules. 2013, 14 (3) pp 881–889. February 1, 2013. DOI: 10.1021/bm3019592.
Sequential Nucleophilic Substitutions Permit Orthogonal Click Functionalization of Multicomponent PEG Brushes. Jin Sha, Ethan S. Lippmann, Jason McNulty, Yulu Ma, and Randolph S. Ashton. Biomacromolecules. 2013, 14 (9) pp 3294–3303. August 13, 2013. DOI: 10.1021/bm400900r.
Detection of O -GlcNAc Modi fi cations on Cardiac Myo fi lament Proteins. Genaro A. Ramirez-Correa , Isabel Martinez Ferrando , Gerald Hart and Anne Murphy. Methods in Molecular Biology. 2013, 1005 (13) pp 157-168. January 1, 2013. DOI: 10.1007/978-1-62703-386-2_13.
Applicable patents and legal notices are available at legal notices.
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