Biotin-dPEG®₁₁-azide (QBD-10784)

Description

Biotin-dPEG®11-azide, product number QBD-10784, is a water-soluble biotinylation product functionalized with azide for click chemistry use, on a medium-length (40 atoms) single molecular weight, discrete PEG (dPEG®) chain. This product enables biotinylation of molecules using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®11-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®11-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

As a biotinylation reagent, Biotin-dPEG®11-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®11-azide. For instance, in a novel use of QBD-10784, Biotin-dPEG®11-azide was employed in a pull-down assay to identify electrophiles in signaling pathways in the worm Caenorhabditis elegans.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), and specifically see pp. 722-724.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Cardiovascular Small Heat Shock Protein HSPB7 Is a Kinetically Privileged Reactive Electrophilic Species (RES) Sensor. Sanjna L. Surya, Marcus J. C. Long, Daniel A. Urul, Yi Zhao, Emily J. Mercer, Islam M. Elsaid, Todd Evans, and Yimon Aye. ACS Chemical Biolody. 2018, 13, pp 1824-1831. February 3, 2018. DOI: 10.1021/acschembio.7b00925.

Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens. Shixian Lin, Zhenrun Zhang, Hao Xu, Lin Li, She Chen, Jie Li, Ziyang Hao, and Peng R. Chen. J. Am. Chem. Soc. 2011, 133 (50), pp 20581–20587. November 15, 2011. DOI: 10.1021/ja209008w.

Precision Electrophile Tagging in Caenorhabditis elegans. Marcus J. C. Long, Daniel A. Urul, Shivansh Chawla, Hong-YuLin, Yi Zhao, Joseph A. Haegele, Yiran Wang, and Yimon Aye. Biochemistry. 2018, 57 (2), pp 216-220. August 31, 2017. DOI: 10.1021/acs.biochem.7b00642.

Binding of the Treslin-MTBP Complex to Specific Regions of the Human Genome Promotes the Initiation of DNA Replication. Akiko Kumagai, William G. Dunphy. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. 2020. Volume 32, Issue 12. 9/22/2020. http://doi.org/10.1016/j.celrep.2020.108178

Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical locales. Yi Zhao, Pierre A Miranda Herrera, Dalu Chang, Romain Hamelin, Marcus J C Long, Yimon Aye. PNAS Biochemistry. 2022. January 26, 2022. https://doi.org/10.1073/pnas.2120687119