BCIP/NBT AP Substrate Kit forms an indigo, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
*Biotechniques (2007) 42:756-759 and BioTechniques (2014) 57:254-256
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Alkaline Phosphatase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Indigo |
| Color of Fluorescence | Far Red |
| Microscopy | Brightfield, Fluorescence, Spectral Imaging |
| Additional Substrate Properties | Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling |
| Mounting | Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent) |
| Safety Title | WARNING: Reproductive Harm – www.P65Warnings.ca.gov |
This substrate can be used for developing blots or for in situ hybridization. At 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. This kit contains stock solutions in convenient dropper bottles.
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