AZDye™ 594 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye structurally is identical to Alexa Fluor® 594. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF® 594 Dye, DyLight® 594 and Alexa Fluor® 594.
Unit Size | 1 mg, 5 mg, 25 mg |
---|---|
Abs/Em Maxima | 590/617 nm |
Extinction Coefficient | 88,000 |
Flow Cytometry Laser Line | 561 nm or 594 nm |
Microscopy Laser Line | 594 nm |
Spectrally Similar Dyes | Alexa Fluor® 594, CF® 594, DyLight® 594 |
Molecular weight | 925.00 (protonated) |
CAS | N/A |
Solubility | Water, DMSO, DMF |
Purity | >95% (HPLC) |
Appearance | Red solid |
Storage Conditions | -20°C. Desiccate |
Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
Stay in the Loop. Join Our Online Community
Products
Ordering
About Us
©Vector Laboratories, Inc. 2024 All Rights Reserved.
How do I Request a Quote?
To request a quote for products: