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AZDye 594 Biotin (CCT-1396)

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Description

AZDye 594 Biotin (Alexa Fluor® 594 Biotin equivalent) can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AZDye 594 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima590/617 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
532 or 594 nm
Microscopy Laser Line
594 nm
Spectrally Similar DyesAlexa Fluor® 594, Atto™ 594, CF™ 594 Dye, DyLight™594
Molecular weight1123.32
CASN/A
SolubilityWater, DMSO, DMF, MeOH
Purity>95% (HPLC)
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB594

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