AZDye™ 594 Azide is a bright, red-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. AZDye™ 594 is well suited for the 561 nm and 594 nm laser lines of the Ar-Kr mixed-gas laser.
AZDye™ 594 Azide dye structurally is identical to Alexa Fluor® 594 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF®594 Dye, DyLight® 594 and Alexa Fluor® 594.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 590/617 nm |
| Extinction Coefficient | 88,000 |
| Flow Cytometry Laser Line | 561 nm or 594 nm |
| Microscopy Laser Line | 594 nm |
| Spectrally Similar Dyes | Alexa Fluor® 594, CF® 594, DyLight® 594 |
| Molecular weight | 846.97 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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