AZDye™ 488 Azide is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor® 488 Azide. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488 and CF® 488 Dye.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||494/517 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
|Spectrally Similar Dyes||FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488|
|Molecular weight||658.66 (protonated)|
|Solubility||Water, DMSO, DMF|
|Appearance||Orange to light red solid|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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