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AZDye™ 532 NHS Ester is an amine reactive, yellow-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.
AZDye™ 532 is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.
Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
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Reactivity | Primary amines |
Abs/Em Maxima | 530/555 nm |
Extinction coefficient | 81,000 cm-1M-1 |
Solubility | Water, DMSO, DMF |
Spectrally similar dyes | Alexa Fluor® 532, DyLight® 532 |
Molecular weight | 723.80 |
Storage Conditions | -20°C. |
Shipping Conditions | Ambient temperature |
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