AZDye™ 532 Azide is a bright, yellow-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 532 Azide is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.
AZDye™ 532 is structurally similar to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 532/554 nm |
| Extinction Coefficient | 78,000 |
| Flow Cytometry Laser Line | 532 nm |
| Microscopy Laser Line | 532 nm |
| Spectrally Similar Dyes | Alexa Fluor® 532, Atto™ 532, CF® 532 dyes |
| Molecular weight | 738.79 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Orange to light red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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