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AZDye 532 Azide (CCT-1279)

[text-backorder]

This product was previously sold as 1043 from Fluoroprobes.

Description

AZDye™ 532 Azide is a bright, yellow-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 532 Azide is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

AZDye™ 532 is structurally similar to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima532/554 nm
Extinction Coefficient
78,000
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Spectrally Similar DyesAlexa Fluor® 532, Atto™ 532, CF® 532 dyes
Molecular weight738.79
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceOrange to light red solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF532

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