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AZDye 430 DBCO (CCT-1274)

[text-backorder]

This product was previously sold as 1036 from Fluoroprobes.

Description

AZDye™ 430 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 430 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.

AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.

AZDye™ 430 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima430/537 nm
Extinction Coefficient
15,000
Spectrally Similar DyesAlexa Fluor® 430, CF® 430
Molecular weight761.81
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceYellow solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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