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Alkaline Phosphatase Avidin D (A-2100-100)



Alkaline Phosphatase Avidin D is produced with the highest specific activity enzymes in optimal ratios. It is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.


  • Specific covalent linkages are chosen to provide stable, highly active conjugates
  • One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 °C, pH 9.8


Unit Size100 U
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended UsageReconstitute by adding 1 ml water. The resulting solution will have the following composition: 10 mM HEPES, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 0.1 mM zinc acetate, pH 7.5, 50 mg/ml Ficoll, and 2 mg/ml bovine serum albumin. Recommended concentration for use is 0.2 units/ml.
Recommended Storage2-8 °C
Detection Enzyme(s)Alkaline Phosphatase
ConjugateAlkaline Phosphatase


Technical Information

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules. Specific covalent linkages are chosen to provide stable, highly active conjugates.

Applicable patents and legal notices are available at legal notices.

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