Amino-dPEG®₈-acid (QBD-10277)

Description

Amino-dPEG®8-acid, product number QBD-10277, is an amino PEG acid. A primary amine and propionic acid terminate the ends of the polyethylene glycol (PEG) spacer. The single molecular weight PEG spacer is discrete (Ð = 1) and highly hydrophilic. Consequently, it increases the hydrodynamic volume and imparts water solubility to conjugates that incorporate it.

Both ends of the molecule are available for conjugation. Conjugation of only one end of the dPEG® spacer may modify the overall charge of the conjugate. Many different types of coupling reactions are effective for this purpose. However, care should be taken not to use single-step EDC coupling, as this will polymerize the dPEG® product.

Scientific publications report the use of Amino-dPEG®8-acid in modifying diverse compounds including antibodies, enzymes, carbon nanotubes, and SU-8 (an epoxy-based negative photoresist) layered atop a quartz surface. In each case, amino-dPEG®8-acid was shown to improve the performance of the conjugate compared to the unmodified material. The improved conjugate performance occurred through increased signal-to-noise ratio, reduced non-specific binding, or both.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Spacer length effects on in vitro imaging and surface accessibility of fluorescent inhibitors of prostate specific membrane antigen. Tiancheng Liu, Jessie R. Nedrow-Byers, Mark R. Hopkins, Clifford E. Berkman. Bioorganic & Medicinal Chemistry Letters. 2011, 21 (23), pp 7013–7016 October 4, 2011. DOI:10.1016/j.bmcl.2011.09.115.

Short PEG-Linkers Improve the Performance of Targeted, Activatable Monoclonal Antibody-Indocyanine Green Optical Imaging Probes. Kohei Sano, Takahito Nakajima, Kiminori Miyazaki, Yuya Ohuchi, Takashi Ikegami, Peter L. Choyke and Hisataka Kobayashi. Bioconjugate Chem. 2013, 24 (5) pp 811–816. April 22, 2013. DOI: 10.1021/bc400050k.

Novel 3D LithographicallyPrepared Solid-Phase Surfaces Made from SU-8 for Next Generation Sequencing. Hong Wang, Makgorzata Witek, Daniel Park, Jianmin Huang,Francis Barany, Steven A. Soper. Miniaturized Systems for Chem and Life Sci. 2011, pp.73-75. October 6, 2011. DOI: www.rsc.org/images/LOC/2011/PDFs/Papers/026_0829.pdf

Drug loading, dispersion stability, and therapeutic efficacy in targeted drug delivery with carbon nanotubes. Elena Heister, Vera Neves, Constanze Lamprecht, S.Ravi P. Silva, Helen M. Coley, Johnjoe McFadden. Carbon. 2011, 50 (2) pp 622-632. August 31, 2011. DOI: 10.1016/j.carbon.2011.08.074.

Enzyme Catalytic Efficiency: A Function of Bio_nano Interface Reactions. Alan S Campbell, Chenbo Dong, Fanke Meng, Jeremy Hardinger, Gabriela C Perhinschi, Nianqiang Wu, and Cerasela Zoica Dinu. ACS Applied Materials and Interfaces. 2014, 6 pp 5393-5403. March 25, 2014. DOI: dx.doi.org/10.1021/am500773g.

Redirecting extracellular proteases to molecularly guide radiosensitizing drugs to tumors. Dina V. Hingorani, Jessica L. Crisp, Matthew K. Doan, Maria F. Camargo, Maryam A. Quraishi, Joseph Aguilera, Mara Gilardi, Larry A.Gross, Tao Jiang, Wei T. Li, Weg M.Ongkeko, Ezra E. W. Cohen, j. Silvio Gutkind, Stephen R. Adams, Sunil J .Advania. Biomaterials. 2020, Volume 248, July 2020, 120032. 04/11/2020. https://doi.org/10.1016/j.biomaterials.2020.120032