Multiplexing Solutions

Diverse Options. Reliable Results.

Unlock Deeper Insights with Advanced Multiplexing Solutions

Multiplex immunostaining is revolutionizing spatial biology, personalized medicine, and translational research by enabling researchers to simultaneously detect multiple biomarkers within a single tissue specimen. By preserving the spatial context of biomolecular interactions, multiplexing provides a comprehensive molecular and cellular view offering unparalleled insights into complex cellular relationships, biomarker expression patterns, and disease mechanisms. Scientists across disciplines—from oncology and neuroscience to regenerative medicine—rely on multiplexing to maximize data acquisition from limited samples, accelerate biomarker discovery, and improve diagnostic precision, ultimately deepening our understanding of tissue biology and disease progression.

Vector Laboratories: Pioneering Solutions for Complex Workflows

Vector Laboratories is committed to advancing multiplex immunostaining methodologies. Our extensive portfolio of detection reagents is designed to address the diverse needs of multiplex staining workflows, ensuring researchers have the tools required to achieve clear, reliable, and reproducible results. In addition to our comprehensive portfolio of detection reagents, Vector has recently launched VectaPlex™, our latest innovation designed to eliminate cross-reactivity between multiplex staining rounds. This ready-to-use kit removes antibodies and other non-covalently bound detection reagents from formalin-fixed paraffin-embedded (FFPE) tissue sections in immunohistochemistry, immunofluorescence, and tyramide signal amplification workflows. VectaPlex streamlines the multiplexing process by reducing assay optimization time while preserving tissue integrity and antigenicity of targets, all while delivering precise marker localization.
Multiplexing Solutions Figure1

Figure 1: Stomach section stained with 6 mouse primary antibodies (CD20 (Red), CD34 (Yellow), DES (Cyan), AE1/AE3 (Magenta), CD3 (Grey), VIM (Green)) detected with DyLight™ 488 horse anti-mouse IgG and mounted with VECTASHIELD® PLUS with DAPI. This composite image was generated from 6 rounds of immunofluorescent staining using the VectaPlex Antibody Removal Kit.

Multiplexing Solutions Figure2

Figure 2: VectaPlex preserves tissue antigenicity and morphology more than (A) pressure cooking or (B) microwave treatment. Images of tissue damage as shown by hematoxylin staining before any treatment (top row), after 6 rounds of pressure cooking/microwave (middle row) and by VectaPlex (bottom row). VectaPlex preserves tissue morphology while other methods show extensive tissue damage.

To support diverse workflow requirements, Vector offers a range of products tailored for multiplex applications:
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VectaPlex Antibody Removal Kit

Remove antibodies and eliminate cross-reactivity between multiplex staining rounds

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Animal-Free Blocker® and Diluent, R.T.U.

Stabilize diluted antibody solutions and diminish background in IHC applications.

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Enzyme Substrates

Visualize target antigens with precise colorimetric staining.

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Mounting Media

Preserve histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.

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Extensive Primary Antibody Portfolio

Select from IHC validated antibodies against 1000s of targets.

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Secondary Antibodies

Experience exceptional specificity and sensitivity across diverse research needs.

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Antibody Engineering Service

Reformat antibodies into different species and isotypes to streamline multiplexing workflows.

Why Choose Vector Laboratories?

Expertise and Innovation

With decades of experience, we continually innovate to provide cutting-edge tools that meet the evolving needs of the scientific community.

Comprehensive Support

Our team offers unparalleled technical assistance, ensuring optimal application of our products in your research.

Quality Assurance

We adhere to stringent quality control measures, guaranteeing the reliability and consistency of our reagents.

As a leader in immunodetection, Vector Laboratories empowers researchers with advanced multiplex IHC solutions. Our commitment to quality, innovation, and support positions us as the partner of choice for scientists seeking to unravel the complexities of tissue biology.

Explore our comprehensive suite of products and discover how we can enhance your multiplex IHC and IF workflows.

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Resources

Immunofluorescence Resource Guide

Streamline the immunohistochemistry workflow with this step-by-step guide for selecting the best reagents for your project.

IHC Multiplexing Resource Guide

Discover the steps and considerations needed for creating stunning multicolor IHC images.

Immunohistochemistry Resource Guide

Step-by-step guide for selecting the best reagents for your immunohistochemistry workflow.