VectaPlex™ Antibody Removal Kit (VRK-1000)

Description

Your path to simplified multiplexing starts with VectaPlex™.  This kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunofluorescence and tyramide signal amplification staining workflows.

Benefits include:

• Obtain more spatial biomarker information from each tissue section.
• Detect 6+ targets in a single tissue using two-color fluorescent imaging and standard IF reagents.
• Multiplex at room temperature with minimal tissue damage or loss of antigenicity.
• Preserve precious sample in IF and TSA, including sensitive tissues such as lung, skin, and breast.
• Stain with multiple primaries from the same species and a single secondary without cross-reactivity.
• Reduce the need for optimization of the sequence in which antibodies are bound during TSA.

The first image above is a composite image generated from individual images taken during six rounds of immunofluorescence staining using VectaPlex.  FFPE stomach; six mouse primary antibodies – CD20 (red), CD34 (yellow), Desmin (cyan), AE1/AE3 (magenta), CD3 (gray), Vimenn (green) – detected with DyLight™ 488 horse anti-mouse IgG.  Blue indicates DAPI stain.  Vector® TrueView® Autofluorescence Quencher Kit applied to suppress background fluorescence.

The second image above illustrates how VectaPlex preserves tissue morphology after several rounds of treatment, unlike protocols requiring heat treatment. FFPE breast carcinoma is shown, stained with hematoxylin & eosin for easy visualization.

The third image above was captured after three rounds of tyramide staining. VectaPlex was used after each round. FFPE tonsil is shown, following application of 3 mouse primary antibodies – CD3 (red), CD68 (green), AE1/AE3 (purple) – detected using Opal 3-plex TSA Manual Detection Kit. Blue indicates DAPI stain.

Specifications

Kit Contents:

• VectaPlex™ Antibody Removal Reagent A
• VectaPlex™ Antibody Removal Reagent B

Documents

Technical Information

This ready-to-use kit is designed to remove antibodies and other non-covalently bound detection reagents from formalin-fixed paraffin-embedded (FFPE) tissue sections in immunofluorescence or tyramide signal amplification staining workflows.

VectaPlex simplifies sample processing for manual workflows, accelerates multiplex assay optimization, preserves precious sample, and maintains tissue integrity and morphology through multiple cycles of antibody binding and removal.