Choice can be a double-edged sword—the freedom and access to a wide range of options to solve the exact problem you’re facing, coupled with what seems like endless possibilities and the challenge of finding the best approach to meet your needs. Faced with these choices you may start feeling overwhelmed.
When it comes to choosing your immunohistochemistry (IHC) detection system there are many considerations and important decisions to be made. Should you use a biotin or non-biotin system? How abundant is your protein of interest? What if your primary antibody species is the same as the tissue? Some decisions may seem inconsequential, but together they combine to give you the perfect picture and proof of your experimental success.
IHC is an established, sensitive method using antibodies to detect specific target antigens (proteins) in tissue sections. IHC exploits antibody-to-antigen affinity and utilizes enzymes for chromogenic reactions. If this is your first foray into IHC or you’re looking to improve your results, the choice of reagents and methods can be mind boggling. When designing your IHC assays, here are some vital considerations that will help you choose the right reagents:
Whew – that was a lot! Take a breath, consider all the points above, and consult the following table to help you choose your detection system:
There are many choices to fit a range of budgets, sensitivities, and performance levels. As pioneers of IHC technology, Vector Laboratories is here to help you choose the correct IHC detection reagents to set you on the path to success and give you confidence in your results.