Immunohistochemistry Resource Guide

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Polymer-Based Detection

Non-biotin micropolymer-based detection for greater signal, low background, and superior access to epitopes

Polymer-based reagents are a more recent introduction into IHC detection methodology than traditional avidin and biotin conjugates, such as ABC kit formats. Polymers offer distinct advantages over these traditional methods particularly for applications such as multiple antigen labeling (multiplexing) on the same tissue section, or in instances where detectable levels of endogenous biotin may be problematic.

Polymer-based systems essentially consist of an integrated polymer of active enzyme and secondary antibody that binds to a primary antibody target. This integrated format introduces significantly more enzyme at the site of localization, thereby generating a greater reaction with the subsequent chromogen, compared with a secondary antibody directly conjugated with enzyme. Additionally, use of a one-step polymer method shortens the IHC procedure by avoiding the two-step biotinylated secondary antibody and ABC reagent that are required for standard avidin-biotin systems.

Polymer-based systems were initially introduced consisting of dextran or similar molecules that had inherent issues in some tissues due to their large size. Our ImmPRESS™ polymer systems have been highly refined and consist of micropolymers that penetrate more easily into thicker sections, avoid steric hindrance concerns, and provide defined, specific binding to the primary antibody.


Small Bowel: • Ki67 (rp), ImmPRESS™ Reagent (HRP; Universal), Vector® NovaRED™ (red) • Cytokeratin 8/18 (m), ImmPRESS™ Reagent (HRP; Universal), Vector® SG (blue-gray).


Breast Carcinoma: • Estrogen Receptor (m), ImmPRESS™ Reagent (HRP; Universal), Vector® DAB (brown) • Cytokeratin AE1/AE3 (m), VECTASTAIN® ABC-AP Kit (Universal), Vector® Red (magenta).


Breast Carcinoma: • Estrogen Receptor (m), ImmPRESS™ Reagent (HRP; Universal), Vector® NovaRED™ HRP Substrate (red) • CD34 (m), ImmPRESS™ Reagent (HRP; Universal), Vector® DAB+Ni HRP Substrate (gray-black).


Small Bowel: • Neurofilament 200 kDa (m), ImmPRESS™ Reagent (HRP) Anti-Mouse IgG, Vector® VIP (purple) • Desmin (m), ImmPRESS™ Reagent (HRP) Anti-Mouse IgG, Vector® SG (blue-gray).


Breast Carcinoma: • Estrogen Receptor (m), ImmPRESS™ Reagent (HRP; Universal), Vector® NovaRED™ (red) • CD34 (m), VECTASTAIN® ABC-AP Kit (Universal), Vector® Blue (blue).


Mouse Tonsil: CD45 (rat), ImmPRESS™ Reagent (HRP) Anti-Rat IgG, Mouse Adsorbed, ImmPACT™ AEC (red), Hematoxylin QS (blue).


Small Bowel: • CD10 (m), ImmPRESS™ Reagent (HRP) Anti-Mouse IgG, Vector® DAB-Ni (black) • Cytokeratin 20 (m), ImmPRESS™ Reagent (HRP) Anti-Mouse IgG, Vector® NovaRED™ (red).

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