Mouse on Mouse (M.O.M.) Kits

For detecting anti-mouse antibodies in mouse tissues

An anti-mouse detection antibody generally cannot distiguish between a primary antibody produced in mouse and the endogenous mouse immunoglobulins present in mouse tissue. Such applications often result in high background staining, which obscures specific staining. Eliminate this problem by using M.O.M. Kits. In addition to the inclusion of our proprietary M.O.M. Mouse Ig Blocking Reagent, these kits offer;

  • A significant reducuction in background staining in mouse tissues
  • Clear, crisp staining of the antigens of interest
  • The avoidance of tedious calculations and primary antibody pre-binding steps

Mouse on Mouse (M.O.M.) Kit options include:

LEFT Image: No M.O.M. Kit utilized. Frozen Mouse Intestine double labeled with Peripherin (m), Biotinylated Horse Anti-Mouse IgG, Fluorescein Avidin DCS (green); and Desmin (m), Biotinylated Horse Anti-Mouse IgG, Texas Red® Avidin DCS (red). Note background and signal mixing.

RIGHT Image: M.O.M. Kit utilized: Frozen Mouse Intestine double labeled with Peripherin (m), M.O.M. Fluorescein Kit (green), and Desmin (m), M.O.M. Basic Kit, Texas Red Avidin DCS (red). Compare with adjacent image prepared without M.O.M. Kit.

Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
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IHC Troubleshooting Guide for ABC Systems

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems.  Also troubleshoot issues of weak or no staining. Laminated.

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IHC Troubleshooting Mouse Primaries on Mouse Tissue

This guide for troubleshooting mouse primary antibodies on mouse tissue describes some common difficulties encountered in this procedure and details strategies for customizing the M.O.M. Kits to address them.

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