Vector Laboratories’ blog provides a wealth of information on immunohistochemistry (IHC), immunofluorescence (IF), and related life science research topics.
Adding multiple antibodies to your IHC protocol can lead to stunning scientific insights, but is sometimes technically challenging. Get answers to your multiplex immunostaining quandaries from Dr. Craig Pow in this tips and tricks article.
Immunohistochemistry (IHC) is an invaluable technique in a variety of fields—from basic research to clinical diagnosis—but using IHC to screen humanized antibodies in human tissue can be challenging. Learn how scientists are addressing those challenges,... Read more
Protein in formalin-fixed tissue can undergo epitope masking, leading to reduction or elimination of antibody binding. Learn how antigen retrieval can reverse this masking.
No matter how you slice it, if your research includes immunohistochemistry or immunofluorescence, it’s a key part of your experimental workflow. If you aren’t the type of researcher who draws a heart around “Sectioning Day”... Read more
Looking for insights into how to choose the right mounting medium for your applications? From understanding the importance of refractive index to technique tips, we’ve got you covered.
Picture this: a chemist walks into a lab and asks his colleagues how to transition some immunohistochemistry (IHC)-stained slides in a buffer solution to coverslip with a non-aqueous mounting media. From his perspective, it’s a... Read more
When scientists detect targets of interest with immunohistochemistry (IHC), antibody binding and substrate detection alone often produce a perplexing picture where the target lacks context. Luckily there’s a solution. Counterstaining IHC samples after enzymatic detection... Read more
In this publication highlight, we review a study examining the impact of macrophage-derived microparticles on the tumor microenvironment and their synergistic effect on anti-PD-1 antibody activity.
When it comes to selecting the substrate for your IHC application, you have variety and flexibility, and our focus is to help you make the right decision. This blog discusses different factors to consider—enzyme, sensitivity,... Read more
When performing IHC, scientists aspire to reduce background or non-specific staining on the specimen. Explore how you can block the sources of non-specific signals increases the signal-to-noise ratio for optimal results.
