Top Considerations for Selecting Immunohistochemistry (IHC) Detection Reagents
Choosing the appropriate immunohistochemistry detection reagent can be a daunting task. Here are some considerations to guide you on your path to staining success.
Insights from our team
Vector Laboratories’ blog provides a wealth of information on immunohistochemistry (IHC), immunofluorescence (IF), and related life science research topics.
Immunohistochemistry
Start Right to Finish Well—The Importance of the Sample Preparation Process in Your IHC and IF Workflow
When it comes to IHC and IF a strong start is essential to great results. Explore our tips to help your sample preparation process.
Easy as Do-Re-Mi: Getting Started with Multiplex Immunohistochemistry
Are you eager to add multiplex immunohistochemistry to your experimental repertoire, but feeling unsure about how to begin? We’re here to help with this tips and tricks article.
Answering Your Questions: Getting Started With Multiplex Immunohistochemistry
Adding multiple antibodies to your IHC protocol can lead to stunning scientific insights, but is sometimes technically challenging. Get answers to your multiplex immunostaining quandaries from Dr. Craig Pow in this tips and tricks article.
Simplifying Tissue Cross-Reactivity: Screening Humanized Antibodies in Human Tissue
Immunohistochemistry (IHC) is an invaluable technique in a variety of fields—from basic research to clinical diagnosis—but using IHC to screen humanized antibodies in human tissue can be challenging. Learn how scientists are addressing those challenges,... Read more
Unmasking Epitopes Using Antigen Retrieval for Formalin-Fixed Tissue Staining
Protein in formalin-fixed tissue can undergo epitope masking, leading to reduction or elimination of antibody binding. Learn how antigen retrieval can reverse this masking.
Let’s Cut to the Chase: Tips and Tricks to Get the Most Out of Your Sectioning
No matter how you slice it, if your research includes immunohistochemistry or immunofluorescence, it’s a key part of your experimental workflow. If you aren’t the type of researcher who draws a heart around “Sectioning Day”... Read more
The End of the Line: Considerations for Mounting Media Selection
Looking for insights into how to choose the right mounting medium for your applications? From understanding the importance of refractive index to technique tips, we’ve got you covered.
The Waiting Game: A Search for a Streamlined IHC Protocol
Picture this: a chemist walks into a lab and asks his colleagues how to transition some immunohistochemistry (IHC)-stained slides in a buffer solution to coverslip with a non-aqueous mounting media. From his perspective, it’s a... Read more
Dial up the Contrast with Counterstaining
When scientists detect targets of interest with immunohistochemistry (IHC), antibody binding and substrate detection alone often produce a perplexing picture where the target lacks context. Luckily there’s a solution. Counterstaining IHC samples after enzymatic detection... Read more
