Vector Laboratories’ blog provides a wealth of information on immunohistochemistry (IHC), immunofluorescence (IF), and related life science research topics.
When scientists detect targets of interest with immunohistochemistry (IHC), antibody binding and substrate detection alone often produce a perplexing picture where the target lacks context. Luckily there’s a solution. Counterstaining IHC samples after enzymatic detection... Read more
In this publication highlight, we review a study examining the impact of macrophage-derived microparticles on the tumor microenvironment and their synergistic effect on anti-PD-1 antibody activity.
When it comes to selecting the substrate for your IHC application, you have variety and flexibility, and our focus is to help you make the right decision. This blog discusses different factors to consider—enzyme, sensitivity,... Read more
When performing IHC, scientists aspire to reduce background or non-specific staining on the specimen. Explore how you can block the sources of non-specific signals increases the signal-to-noise ratio for optimal results.
There’s nothing worse than toiling away all week to repeat an experiment only to have your staining turn up blank. You’re left scratching your head trying to figure out why you’ve got nothing. Recently, Vector... Read more
Immunohistochemistry Immunofluorescence Immunohistochemistry (IHC) and immunofluorescence (IF) are widely used across the life sciences—in cancer research, immunology, neuroscience, cell biology, and histopathology—to help elucidate protein expression in cell and tissue specimens. And while these are... Read more
