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VectaFluor™ Horse Anti-Rabbit IgG, DyLight™ 594 Antibody Kit, R.T.U. (DI-1794-15)

The product has been discontinued

Please consider the following as potential substitutes: DI-1094-1.5. Horse Anti-Rabbit IgG Antibody (H+L), DyLight 594. Or contact Technical Support @ [email protected] for assistance in finding alternative.

Description

VectaFluor R.T.U. DyLight 594 Anti-Rabbit IgG can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Ready-to-use format offers maximum convenience for fluorescence staining of cells and tissues
  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with DyLight 594 to provide the brightest label for fluorescence microscopy 
  • Excitation: 592 nm 
  • Emission: 617 nm 
  • Color: Red

 

Kit Contents:

  • 15 ml pre-diluted, stabilized secondary antibody solution 
  • 15 ml 2.5% normal horse serum for blocking

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Maximum Emission617 nm
Maximum Excitation592 nm
Target SpeciesRabbit
ConjugateDyLight 594
Color of FluorescenceRed
Host SpeciesHorse
FormatReady-to-Use

Citations

Technical Information

VectaFluor Ready-to-Use (R.T.U.) DyLight dye-labeled Secondary Antibodies offer maximum convenience for fluorescence staining of cells and tissues. Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity.

VectaFluor R.T.U. Antibody Reagents are supplied as 15 ml pre-diluted, stabilized, ready-to-use solutions along with 15 ml ready-to-use 2.5% normal horse serum for blocking.

DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red).

Dylightspectra Jpg Dylightspectra Vectorlabs
Dylight Table Jpg Dylight table Vectorlabs

Applicable patents and legal notices are available at legal notices.