Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.

UBQ-2 NHS Ester (FP-1741)

[text-backorder]

Description

Universal Black 2 Quencher (UBQ-2, Black Hole Quencher®-2 (BHQ®-2) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 560-670 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes such as Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, and IRDye® 650.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima580 nm
Extinction coefficient38,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesCy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, IRDye® 650
Molecular weight603.59
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Ub Ub

Protein Labeling Calculator

[protein-labeling-calculator]

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Log in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote