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Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.
TFP-dPEG®12-biotinidase-resistant biotin, product number QBD-10204, is a water-soluble biotinylation reagent modified to resist hydrolysis by biotinidase (EC 3.5.1.12). This product combines Biotinoyl-2-aminobutyric acid with the 2,3,5,6-tetrafluorophenyl (TFP) ester of a medium-length, single molecular weight, discrete polyethylene glycol (dPEG®) linker. TFP-dPEG®12-biotinidase-resistant biotin retains biotin’s typical high affinity for avidin and streptavidin but also blocks the action of biotinidase on biotin. This product is useful in applications such as ELISAs of tissue samples and antibody pretargeting, where endogenous biotinidase can recycle biotin.
Biotinidase is an amidohydrolase that recycles biotin in vivo by breaking the covalent bond between biotin and amino acids such as lysine (i.e., biocytin). Although necessary for healthy bodily function, biotinidase potentially creates problems when biotinylated proteins are used in serological assays, since endogenous biotinidase removes the biotin from these proteins.
Dr. D. Scott Wilbur and colleagues at the University of Washington in Seattle developed biotinidase-resistant biotin in 2006 to facilitate in vivo antibody pretargeting. To improve the product’s water solubility and ability to reach the binding pocket of avidin, a medium-length amino-dPEG®12-propionic acid linker was conjugated to the biotinidase-resistant biotin and subsequently functionalized as an TFP ester.
Like our other dPEG® biotinylation products, TFP-dPEG®12-biotinidase-resistant biotin, QBD-10204, will not cause aggregation and precipitation of biomolecules to which it is conjugated, and the dPEG® spacer reduces non-specific binding. It is potentially beneficial for applications using serological fluids that contain endogenous biotinidase. Useful roles for TFP-dPEG®12-biotinidase-resistant biotin include the following:
therapeutic applications such as antibody pretargeting with biotin-streptavidin systems;
supramolecular constructs (e.g., nanoparticles) used in vivo that depend on biotin-(strept)avidin affinity to maintain structural integrity; and,
ELISAs of serological fluids and tissue samples using biotin-(strept)avidin affinity.
| Unit Size | 100 mg, 1000 mg |
|---|---|
| Molecular Weight | 1077.19; single compound |
| Chemical formula | C₄₇H₇₆F₄N₄O₁₇S |
| CAS | N/A |
| Purity | > 96% |
| Spacers | dPEG® Spacer is 43 atoms and 50.1 Å, resp. |
| Shipping | Ambient |
| Typical solubility properties (for additional information contact Customer Support) | DCM or Acetonitrile |
| Storage and handling | -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure. |
Applicable patents and legal notices are available at legal notices.
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