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Sulfo-Cy5 NHS ester (FP-1675)

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Description

Sulfo-Cy5 dye is one of the most popular far-red fluorescent dyes. It is often a reagent of choice for protein and peptide labeling. Sulfo-Cy5 dye is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. Sulfo-Cy5 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Sulfo-Cy5 conjugates are recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AZDye 647 NHS Ester analog, or IR 650 NHS Ester, which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima648/671 nm
Extinction coefficient250,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 647, DyLight® 649,
Molecular weight847.97
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy5

Protein Labeling Calculator

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