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Phaseolus Vulgaris Leucoagglutinin (PHA-L), Rhodamine (RL-1112-2)

The product has been discontinued

Please consider the following as potential substitutes: FL-1111-2. Phaseolus Vulgaris Leucoagglutinin (PHA-L), Fluorescein. Or contact Technical Support @ [email protected] for assistance in finding alternative.

Description

Phaseolus vulgaris agglutinin is the name ascribed to a family of lectins, each of which consists of four subunits. There are two different types of subunits. One appears to be involved primarily in red cell agglutination and has been designated the “E” subunit (for erythroagglutinin). The other type is involved in lymphocyte agglutination and mitogenic activity and has been termed the “L” subunit (for leucoagglutinin). These subunits combine to produce five isolectins.  PHA-L, with four “L” type subunits, does not agglutinate red cell but is a potent mitogen.

Rhodamine labeled PHA-L has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.

Specifications

Unit Size2 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation545-555 nm
Maximum Emission570-580 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration2 mg active conjugate/ml
ConjugateRhodamine
Color of FluorescenceRed
Sugar SpecificityGalactose, Complex Structures

Citations

Technical Information

PHA-L, with four L type subunits, does not agglutinate red cells but is a potent mitogen. The other three isolectins, designated E3L1, E2L2, and E1L3, have erythroagglutinating and mitogenic activities proportional to the number of respective E or L subunits. We have termed the mixture of the five isolectins PHA (E+L). 

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Elution: 100 mM acetic acid

Applicable patents and legal notices are available at legal notices.