This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
Rhodamine labeled Dolichos biflorus agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 575 nm and the emission maximum is at 600 nm.
Unit Size | 2 mg |
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Applications | Immunofluorescence, Glycobiology |
Recommended Usage | The recommended concentration range for use is 5-20 µg/ml. |
Recommended Storage | 2-8 °C |
Maximum Excitation | 545-555nm |
Maximum Emission | 570-580 nm |
Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 |
Concentration | 2 mg active conjugate/ml |
Conjugate | Rhodamine |
Color of Fluorescence | Red |
Sugar Specificity | N-Acetylgalactosamine |
Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size. This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
Rhodamine labeled Dolichos biflorus agglutinin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.
Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
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