NHS-S-S-dPEG®₄-biotin (cleavable) (QBD-10194)

Description

NHS-S-S-dPEG®4-biotin, product number QBD-10194, combines biotin with the highly useful properties of single molecular weight discrete polyethylene glycol (dPEG®), a cleavable disulfide linker, and the amine-reactive functionality of N-hydroxysuccinimidyl (NHS) ester. The cleavage point for the molecule is well away from the binding pocket for avidin and streptavidin to allow easy optimization of cleavage conditions. NHS-S-S-dPEG®4-biotin has numerous applications, including
antibody-antigen purification,
isolating antibody inhibitors,
generating synthetic antibodies,
determining how cells sense voltage changes,
developing a noncompetitive immunocomplex immunoassay, and
developing a process to identify GTPase inhibitors through quenching resonance energy transfer (QRET).

Specifications

Documents

References

1. Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his chapter 18 on discrete PEG compounds for pegylation applications.
2. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
3. Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins). Sultana Akter, Markus Vehniainen, Lisa Spoof, Sonja Nybom, Jussi Meriluoto, and Urpo Lamminmaki. Analytical Chemistry. 2016, September 22, 2016. DOI: 10.1021/acs.analchem.6b02470.
4. Thiol-Mediated Anchoring of Ligands to Self-Assembled Monolayers for Studies of Biospecific Interactions. Kunal V. Gujraty, Randolph Ashton, Sridhar R. Bethi, Sandesh Kate, Christopher J. Faulkner, G. Kane Jennings, and Ravi S. Kane. Langmuir. 2006, 22 (24) pp 10157–10162. October 19, 2006.DOI: 10.1021/la0621463.
5. Noncovalent Assembly of Anti-Dendritic Cell Antibodies and Antigens for Evoking Immune Responses In Vitro and In Vivo. Anne-Laure Flamar, Sandra Zurawski, Felix Scholz, Ingrid Gayet, Ling Ni, Xiao-Hua Li, Eynav Klechevsky, John Quinn, SangKon Oh, Daniel H. Kaplan, Jacques Banchereau and Gerard Zurawski. J Immunol. 2012, 189 pp 2645-2655. September 1, 2012. DOI: 10.4049/jimmunol.1102390
6. Site-Specific Labeling of DNA and RNA Using an Efficiently Replicated and Transcribed Class of Unnatural Base Pairs. Young Jun Seo, Denis A. Malyshev, Thomas Lavergne, Phillip Ordoukhanian, and Floyd E. Romesberg . J. Am. Chem. Soc. 2011, 133 (49) pp 19878–19888. October 8, 2011. DOI: 10.1021/ja207907d.
7. Biotinylation reagents for the study of cell surface proteins. Giuliano Elia. Proteomics. 2008, 8 (19) pp 4012-4024. April 16, 2008. 10.1002/pmic.200800097.
8. From Unspecific Quenching to Specific Signaling: Functional GTPase Assays Utilizing Quenching Resonance Energy Transfer (QRET) Technology.
9. Kari Kopra. University of Turku. 2015, pp 1-66. April 1, 2015. ISBN 978-951-29-6080-4.
10. Silicone Implants Immobilized with Interleukin-4 Promote the M2 Polarization of Macrophages and Inhibit the Formation of Fibrous Capsules. Hyun-Seok Kim, Seongsoo Kim, Byung-Ho Shin, Chan-Yeong Heo, Omar Faruq, Le Thi Van Anh, Nilsu Dönmez, Pham Ngox Chien, Dong-Sik Chien, Sun-Young Nam, Rong-Min Baek. Polymers. 2021. August 7, 2021. https://doi.org/10.3390/polym13162630