Vector Labs is closed to observe Independence Day (US Holiday) on Thursday, July 4th.
We will be back in the office on Friday. We will respond to emails upon our return. Have a wonderful day.

M.O.M.® (Mouse on Mouse ) Elite® Immunodetection Kit, Peroxidase (PK-2200)

[text-backorder]

Description

The M.O.M. (Mouse on Mouse ) Elite Peroxidase Kit significantly reduces endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue. It is based on VECTASTAIN® ABC reagent protocols.

Key advantages of the Vector M.O.M. Immunodetection Kits:

  • Clear, crisp specific staining of antigens of interest
  • Procedures are simple and easy to follow
  • No tedious calculations or antibody prebinding steps required

 

The Vector M.O.M. Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue sections.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Blotting Applications
TechnologyABC Elite (Avidin/Biotin) Systems, Mouse on Mouse (M.O.M.) Systems
Detection Enzyme(s)Peroxidase
Target SpeciesMouse
FormatConcentrate
Blocking ActionEndogenous Mouse Ig

Kit Contents:

  • 6 ml of M.O.M. Protein Concentrate
  • 1 ml M.O.M. Mouse Ig Blocking Reagent
  • 0.1 ml M.O.M. Biotinylated Anti-Mouse IgG Reagent
  • VECTASTAIN Elite ABC Reagents A(1 ml) and B (1 ml)

Product FAQs

Vector Laboratories’ Mouse on Mouse detection kits (M.O.M.®) are designed for the detection of mouse IgG primary antibodies on mouse tissue sections. For detecting mouse primary antibodies on rat tissue, we offer a selection of anti-mouse, rat adsorbed secondary detection reagents. These are intended to be used for this application and would generate the most optimal signal to noise staining ratio. We offer a biotinylated anti-mouse IgG, rat adsorbed secondary antibody (Cat. No. BA-2001) for use with VECTASTAIN® ABC kits or avidin and streptavidin enzyme conjugates. Alternatively, we offer an ImmPRESS® HRP polymer anti-mouse IgG, rat adsorbed detection kit (Cat. No. MP-7422) for a convenient, one-step IHC methodology.

We do not recommend freezing the ABC kits. In particular, the ABC reagents exhibit a decrease in enzyme activity and lose some sensitivity when stored at – 20 °C for extended periods.

Citations

Technical Information

The Vector M.O.M. Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector M.O.M. Elite Kit. The result is clear, crisp specific staining of the antigens of interest.

The Vector M.O.M. Elite Kit contains a proprietary Mouse IgG Blocking Reagent and is based on the simple and easy to follow VECTASTAIN® ABC reagent protocols; no tedious calculations or antibody pre-binding steps are required. Excellent staining results for a once difficult application have now become routine with the Vector M.O.M. System.

The Vector M.O.M. Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.  The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, “Discovery Through Color: A Guide to Multiple Antigen Labeling” , available on our website.

Four Vector M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.

The Vector M.O.M. ImmPRESS Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS Peroxidase Polymer reagent.

Choose from a wide variety of peroxidase substrates available for chromogenic development.

Product review on Biocompare.com.

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Sign in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote