MAL-dPEG®2-acid, product number QBD-10265, is a crosslinker that joins sulfhydryl groups to free amines through a single molecular weight polyethylene glycol (PEG) chain of discrete length (dPEG®). The maleimidopropyl moiety reacts with sulfhydryl groups via a Michael addition reaction. The propionic acid terminus of the molecule reacts directly with free amines using EDC or another carbodiimide to form amide bonds. Alternatively, activation of the propionic acid group with N-hydroxysuccinimide (NHS), 2,3,5,6-tetrafluorophenol (TFP), or some other acylating agent permits amide bond formation under mild, controlled conditions.
| Unit Size | 100 mg, 1000 mg |
|---|---|
| Molecular Weight | 328.32; single compound |
| Chemical formula | C₁₄H₂₀N₂O₇ |
| CAS | 756525-98-1 |
| Purity | > 98% |
| Spacers | dPEG® Spacer is 16 atoms and 17.5 Å |
| Shipping | Ambient |
| Typical solubility properties (for additional information contact Customer Support) | Methylene chloride, Acetonitrile, DMAC or DMSO. |
| Storage and handling | -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure. |
Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SMCC or sulfo-SMCC on pp. 283-286.
Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
Synthesis and anti-HIV activity of trivalent CD4-mimetic miniproteins. Hengguang Li, Yongjun Guan, Agnieszka Szczepanska, Antonio J. Moreno-Vargas, Ana T. Carmona, Inmaculada Robina, George K. Lewis and Lai-Xi Wang. Bioorganic & Medicinal Chemistry. 2007, 15 (12) pp 4220–4228. June 15, 2007. doi.org/10.1016/j.bmc.2007.03.064.
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