m-dPEG®4-NHS ester, product number QBD-10211, is a short (14 atoms, 15.6 Å), methyl-terminated, discrete length polyethylene glycol (dPEG®) spacer designed for the modification of surfaces containing accessible free amines. The carboxyl terminus of the dPEG® spacer is activated with N-hydroxysuccinimide (NHS) and reacts optimally with free amines at pH 7.0 – 7.5. The rate of hydrolysis of the active ester to the carboxylic acid accelerates with increasing pH. Please note that modification of surface amines on biomolecules (e.g., proteins and peptides) with this uncharged, methyl-capped dPEG® spacer may alter the overall charge of the resulting conjugates.
Numerous applications employ this product. The applications in which this product has been successfully employed include:
cell surface engineering;
surface modification of biomolecules;
coating of carbon nanotubes, quantum dots, and other hydrophobic surfaces;
acoustic sensor development;
diagnostic films for pathogen detection, and,
various imaging applications.
m-dPEG®4-NHS ester, product number 10211, is just one member of an extensive line of methyl-terminated dPEG® products that includes dPEG® spacers containing 2 to 49 ethylene glycol units.
| Unit Size | 100 mg, 1000 mg |
|---|---|
| Molecular Weight | 333.33; single compound |
| Chemical formula | C₁₄H₂₃NO₈ |
| CAS | 622405-78-1 |
| Purity | > 98% |
| Spacers | dPEG® Spacer is 14 atoms and 15.600 Å |
| Shipping | Ambient |
| Typical solubility properties (for additional information contact Customer Support) | Methylene chloride, Acetonitrile, DMAC or DMSO. |
| Storage and handling | -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure. |
Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
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Tuning surface functionalities of Sub-10 nm-sized nanocarriers to target outer retina in designing drug delivery agents for intravitreal administration. Suyeon You, Hyoungtai Kim, Hye-youn Junga, Boram Kim, Eun Jung Lee, Jin Woo Kim, Yoonkyung Kim. Biomaterials. 2020. DOI: 10.1016/j.biomaterials.2020.120188
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