m-dPEG®12-MAL, product number QBD-10289, is a sulfhydryl-reactive, single molecular weight, with a discrete chain length PEG (dPEG®). This product modifies surfaces and biomolecules with free thiol groups through the maleimide reactive group. The non-immunogenic, methyl-terminated dPEG® chain has no charge but adds water solubility and hydrodynamic volume to molecules to which it is conjugated.
The reaction of the maleimide end of m-dPEG®12-MAL, product number QBD-10289, with a sulfhydryl proceeds best at pH 6.5 – 7.5. Conduct the conjugation at the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which creates confusing results. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.
Published uses for m-dPEG®12-MAL include probing the molecular structure and function of proteins and coating nanoparticle surfaces. In particular, studies of molecular structure and function of proteins would not be possible with traditional, dispersed PEG products because the dispersed PEGs would have created an indecipherable mixture of conjugation products. Only a dPEG® product could work in this application.
Unit Size | 100 mg, 1000 mg |
---|---|
Molecular Weight | 710.81; single compound |
Chemical formula | C₃₂H₅₈N₂O₁₅ |
CAS | 88504-24-9 |
Purity | > 98% |
Spacers | dPEG® Spacer is 44 atoms and 50.7 Å |
Shipping | Ambient |
Typical solubility properties (for additional information contact Customer Support) | Methylene chloride, Acetonitrile, DMAC or DMSO. |
Storage and handling | -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure. |
Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). See all of the extensive references to the reactions of maleimides with a variety of compounds and applications, Hermanson, p.1174 (index for maleimides) and references therein.
Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
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