The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product.
The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.
Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.
| Unit Size | 1 Kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs |
| Technology | Micropolymer Reagents |
| Detection Enzyme(s) | Peroxidase |
| Conjugate | Micropolymer HRP |
| Target Species | Universal (Mouse/Rabbit) |
| Format | Ready-to-Use |
The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.
The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.
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