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DNP-dPEG®₄-NHS ester (QBD-10347)



DNP-dPEG®4-NHS ester, product number QBD-10347, consists of a short (16 atoms) single molecular weight, discrete-chain-length (dPEG®) linker functionalized with a 2,4-dinitrophenyl group on one end and a N-hydroxysuccinimidyl (NHS) ester on the other. The NHS ester reacts optimally with free amines in the pH 7.0 – 7.5 range. The fluorescent label 2,4-dinitrophenol, also known as nitrophen, when attached to a non-immunogenic PEG spacer, functions as a hapten. Due to its strong immune response, it may be useful in designing vaccines or in other immunotherapeutic applications.


Unit Size100 mg, 1000 mg
Molecular Weight528.47; single compound
Chemical formulaC₂₁H₂₈N₄O₁₂
Purity> 98%
SpacersdPEG® Spacer is 16 atoms and 18.0 Å
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.


Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). See his chapter 19 especially in hapten-carrier chemistry and applications, as well as Chapter 18 on Discrete PEG pegylation reagents, with much of the chapter featuring our reagents.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Applicable patents and legal notices are available at legal notices.

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