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Cy3 Tyramide (CCT-1545)

SKU Unit Size Price
CCT-1545
150 slides, 1500 slides
$173.00$683.00

Quantity

SKU Package size PriceQuantity
CCT-1545-1 150 slides $173.00
CCT-1545-2 1500 slides $683.00
SKU Unit Size Price
CCT-1545
150 slides, 1500 slides
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Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

Cy3 Tyramide is a component of many tyramide signal amplification kits. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size150 slides, 1500 slides
Abs/Em Maxima555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar DyesAlexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight735.91 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

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