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This biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN® Elite ABC Kit (Universal; PK-6200) and VECTASTAIN® ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN®Universal Quick Kit, PK-8800.
Unit Size | 2.1 mg |
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Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs |
Concentration | 1.05 mg/ml |
Recommended Storage | 2-8 °C; Store frozen for long term storage |
Solution | 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. |
Recommended Usage | The recommended concentration range for use is 10-20 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Note: This product is the same solution as that provided in the VECTASTAIN® Universal ABC Elite® kit (cat. no. PK-6200) and VECTASTAIN® Universal ABC-AP kit (cat. no. AK-5200). |
Target Species | Universal (Mouse/Rabbit) |
Conjugate | Biotinylated |
Host Species | Horse |
Format | Concentrate |
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