Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.
Biotinylated Maackia amurensis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology |
| Recommended Usage | Reconstitute in 1 ml water. The resulting solution will be: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% NaN3, 0.1 mM CaCl2, 0.01 mM MnCl2. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Inhibiting and/or Eluting Sugar | 200 mM lactose (S-9004). |
| Conjugate | Biotinylated |
| Sugar Specificity | Galactose, Lactose |
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011
Inhibiting Sugar: 200 mM lactose
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