Maackia Amurensis Lectin I (MAL I), Biotinylated (B-1315-2)

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Description

Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.

Biotinylated Maackia amurensis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageReconstitute in 1 ml water. The resulting solution will be: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% NaN3, 0.1 mM CaCl2, 0.01 mM MnCl2.
Recommended Storage2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting Sugar200 mM lactose (S-9004).
ConjugateBiotinylated
Sugar SpecificityGalactose, Lactose

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This lectin is the leucoagglutinin or mitogenic isolectin from Maackia seeds.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibiting Sugar: 200 mM lactose

Applicable patents and legal notices are available at legal notices.

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