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Goat Anti-Chicken IgY Antibody (H+L), Biotinylated (BA-9010-1.5)

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Description

The biotinylated goat anti-chicken IgY secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with chicken IgY and IgG primary antibodies
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 

Specifications

Unit Size1.5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration1.5 mg active conjugate/ml
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended UsageThe recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesChicken
ConjugateBiotinylated
Host SpeciesGoat
FormatConcentrate

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used with any avidin or streptavidin detection system for tissue and cell staining, ELISAs, and blots.

Biotinylated Goat Anti-Chicken IgY (H+L) is supplied in solution form. With some exceptions the recommended dilution for most applications is 1:200.

Applicable patents and legal notices are available at legal notices.

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