Biotin-dPEG®3-TFPA, product number QBD-10308, is designed for inserting a biotin label into otherwise inaccessible locations using photoaffinity labeling. The reactive group is the tetrafluorophenyl azide (TFPA), which inserts into random C-H bonds following activation with long-wavelength ultraviolet (UV) light (360 nm, UVA). The short single molecular weight, discrete chain-length PEG (dPEG®) spacer imparts hydrophilicity to biotin, which is normally not very soluble in aqueous media.
The biotin moiety binds tightly with avidin and streptavidin and has proven useful in several different types of applications, including:
affinity chromatography;
construction of supramolecular platforms for various purposes, including drug delivery;
in vivo proximity labeling of proteins and nucleic acids; and,
development and testing of microarray diagnostic sensors.
| Unit Size | 50 mg, 100 mg |
|---|---|
| Molecular Weight | 663.69; single compound |
| Chemical formula | C₂₇H₃₇F₄N₇O₆S |
| CAS | 1264662-85-2 |
| Purity | > 98% |
| Spacers | dPEG® Spacer is 15 atoms and 16.9 Å |
| Shipping | Ambient |
| Typical solubility properties (for additional information contact Customer Support) | DMAC or DMSO. |
| Storage and handling | -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure. |
Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
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Lignin Nanotubes As Vehicles for Gene Delivery into Human Cells. Elena Ten, Chen Ling, Yuan Wang, Arun Srivastava, Luisa Amelia Dempere, and Wilfred Vermerris. Biomacromolecules. 2013, 15 (1) pp 327-338. December 5, 2013. DOI: 10.1021/bm401555p.
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Evidence that C9ORF72 Dipeptide Repeat Proteins Associate with U2 snRNP to Cause Mis-splicing in ALS/FTD Patients. Shanye Yin, Rodrigo Lopez-Gonzalez, Ryan C. Kunz, Jaya Gangopadhyay, Carl Borufka, Steven P. Gygi, Fen-Biao Gao, and Robin Reed. Cell Reports. 2017, 19 (11) pp 2244-2256. June 13, 2017. DOI: 10.1016/j.celrep.2017.05.056.
Close-up of the Immunogenic α1,3-Galactose Epitope as Defined by a Monoclonal Chimeric Immunoglobulin E and Human Serum Using Saturation Transfer Difference (STD) NMR. Melanie Plum, Yvonne Michel, Katharina Wallach, Tim Raiber, Simon Blank, Frank I. Bantleon, Andrea Diethers, Kerstin Greunke, Ingke Braren, Thomas Hack, Bernd Meyer, and Edzard Spillner. The Journal of Biological Chemistry. 2011, 286 (50) pp 43103-43111. December 16, 2011. DOI: 10.1074/jbc.M111.291823.
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A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity. Shuantao Liu, Shuwei Liu, Mei Wang, Tiandi Wei, Chen Meng, Meng Wang, and Guangmin Xia. The Plant Cell. 2014, pp 1-17. January 17, 2014. DOI: 10.1105/tpc.113.118687.
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