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Biotin-dPEG®₃-TFPA (QBD-10308)



Biotin-dPEG®3-TFPA, product number QBD-10308, is designed for inserting a biotin label into otherwise inaccessible locations using photoaffinity labeling. The reactive group is the tetrafluorophenyl azide (TFPA), which inserts into random C-H bonds following activation with long-wavelength ultraviolet (UV) light (360 nm, UVA). The short single molecular weight, discrete chain-length PEG (dPEG®) spacer imparts hydrophilicity to biotin, which is normally not very soluble in aqueous media.

The biotin moiety binds tightly with avidin and streptavidin and has proven useful in several different types of applications, including:
affinity chromatography;
construction of supramolecular platforms for various purposes, including drug delivery;
in vivo proximity labeling of proteins and nucleic acids; and,
development and testing of microarray diagnostic sensors.


Unit Size50 mg, 100 mg
Molecular Weight663.69; single compound
Chemical formulaC₂₇H₃₇F₄N₇O₆S
Purity> 98%
SpacersdPEG® Spacer is 15 atoms and 16.9 Å
Typical solubility properties (for additional information contact Customer Support)DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.


Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Molded hyaluronic-acid gel as a micro-template for blood capillaries. Ko Sugibayashi, Yoshikazu Kumashiro, Tatsuya Shimizu, Jun Kobayashi, and Teruo Okano. Journal of biomaterials science. Polymer edition, 2012 May 22, 2012. DOI: 10.1163/156856212X627847.

Elastomeric microparticles for acoustic mediated Bioseparations. Leah M Johnson, Lu Gao, C Wyatt Shields IV, Margret Smith, Kirill Efimenk, Kevin Cushing, Jan Genzer and Gabriel P López. Journal of Nanobiotechnology. 2013, 11 (22) pp 1-8. June 28, 2013. DOI:10.1186/1477-3155-11-22.

Photo-lithography and soft-lithography of Perfluoropolyethers for the development of fouling release surfaces with biomolecular patterns. Stefano Turri. Politecnico di Milano. 2013, pp vii-121. December 18, 2013. School of Industrial and Information Engineering; Master of Science in Materials Engineering and Nanotechnology; Department of Chemistry, Materials and Chemical Engineering “Guilio Natta” Master’s Thesis –

Detection techniques for biomolecules using semi-conductor nanocrystals and magnetic beads as labels. Esha Chatterjee. Scholars Archive@OSU. 2011, pp 1-146. November 7, 2011. Graduate Oregon State University Thesis/Dissertation –

Biotinylated PLGA nanoparticles as versatile targeting vehicles for anti-cancer drug delivery. Olivia Donaldson, Lucille Bell, Erin Wagner, and Dr. Noelle Comolli. Department of Chemical Engineering, Villanova University. 2011, 719 pp 1.

Lignin Nanotubes As Vehicles for Gene Delivery into Human Cells. Elena Ten, Chen Ling, Yuan Wang, Arun Srivastava, Luisa Amelia Dempere, and Wilfred Vermerris. Biomacromolecules. 2013, 15 (1) pp 327-338. December 5, 2013. DOI: 10.1021/bm401555p.

Platinum plasmonic nanostructure arrays for massively parallel single-molecule detection based on enhanced fluorescence measurements. Toshiro Saito, Satoshi Takahashi, Takayuki Obara, Naoshi Itabashi, and Kazumichi Imai. Nanotechnology. 2011, 22 (44) pp 1-8. October 11, 2011. DOI: 10.1088/0957-4484/22/44/445708.

Evidence that C9ORF72 Dipeptide Repeat Proteins Associate with U2 snRNP to Cause Mis-splicing in ALS/FTD Patients. Shanye Yin, Rodrigo Lopez-Gonzalez, Ryan C. Kunz, Jaya Gangopadhyay, Carl Borufka, Steven P. Gygi, Fen-Biao Gao, and Robin Reed. Cell Reports. 2017, 19 (11) pp 2244-2256. June 13, 2017. DOI: 10.1016/j.celrep.2017.05.056.

Close-up of the Immunogenic α1,3-Galactose Epitope as Defined by a Monoclonal Chimeric Immunoglobulin E and Human Serum Using Saturation Transfer Difference (STD) NMR. Melanie Plum, Yvonne Michel, Katharina Wallach, Tim Raiber, Simon Blank, Frank I. Bantleon, Andrea Diethers, Kerstin Greunke, Ingke Braren, Thomas Hack, Bernd Meyer, and Edzard Spillner. The Journal of Biological Chemistry. 2011, 286 (50) pp 43103-43111. December 16, 2011. DOI: 10.1074/jbc.M111.291823.

Cell labeling and proximity dependent biotinylation with engineered monomeric streptavidin. Jasdeep K. Mann, Daniel Demonte, Christopher M. Dundas, and Sheldon Park. Technology. 2016, 4 (3) pp 152-158. January 25, 2016. DOI: 10.1142/S2339547816400057.

An integrated experimental and modeling approach to propose biotinylated PLGA microparticles as versatile targeting vehicles for drug delivery. Olivia Donaldson, Zuyi Jacky Huang, and Noelle Comolli. Progress in Biomaterials. 2013, 2 (3) pp 1-10. February 13, 2013. DOI: 10.1186/2194-0517-2-3.

A Wheat SIMILAR TO RCD-ONE Gene Enhances Seedling Growth and Abiotic Stress Resistance by Modulating Redox Homeostasis and Maintaining Genomic Integrity. Shuantao Liu, Shuwei Liu, Mei Wang, Tiandi Wei, Chen Meng, Meng Wang, and Guangmin Xia. The Plant Cell. 2014, pp 1-17. January 17, 2014. DOI: 10.1105/tpc.113.118687.

A microfluidic sensor based on ferromagnetic resonance induced in magnetic bead labels. Esha Chatterjee, Tim Marr, Pallavi Dhagat, and Vincent T. Remcho. Sensors and Actuators B: Chemical. 2011, 156 (2) pp 651-656. March 11, 2011. DOI: 10.1016/j.snb.2011.02.012.

Inhibition of Drug-Induced Liver Injury in Mice Using a Positively Charged Peptide That Binds DNA. Pedro E. Marques, Sofie Vandendriessche, Thiago H.C. de Oliveira, Helena Crijns, Mateus E. Lopes, Marfa Blanter, Sara Schuermans, Karen Yu, Fariba Poosti, Vincent Vanheule, Rik Janssens, Daiane Boff, Andreas J. Kungl, Gustavo B. Menezes, Mauro M. Teixeira, Paul Proost. Hepatology Communications. 2021, Volume 5, Issue 10. 10/2021. DOI: 10.1002/hep4.1759

Applicable patents and legal notices are available at legal notices.

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