Biotin-dPEG®₁₁-MAL (QBD-10195)

Description

Biotin-dPEG®11-MAL, product number QBD-10195, is one of three products containing a biotin on one end of a dPEG® linker and a maleimidopropyl moiety on the other end. The maleimide group is a useful, highly popular reactive group for conjugating to free thiol groups in biomolecules. This product provides a short-chain, single molecular weight, discrete PEG (dPEG®) spacer between the biotin and the maleimide. Maleimide groups can be conjugated to free thiol groups in the pH range from 6.5 – 7.5, forming a stable thioether linkage. The dPEG® spacer in this product increases the hydrophilicity of the conjugate.

This product has proven useful in a variety of applications that take advantage of the high streptavidin-biotin binding affinity. Such applications include magnetic bead-based assays, assays to quantitate reducible thiols in proteins, atomic force microscopy, and nanoparticle applications, among others.

To use Biotin-dPEG®11-MAL, the product should be dissolved in dry solvent and then added to an aqueous solution of the compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent to use for this purpose. For biomolecules (e.g., antibodies), care should be taken to keep the amount of organic solvent to a minimum so as not to denature the biomolecule or cause salt precipitation.

Specifications

Documents

References

1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
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