BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) (SK-5400)

Prostate: Psa (m), Vectastain Abc-ap Mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy. Prostate: Psa (m), Vectastain Abc-ap Mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy. Vectorlabs
Prostate: Psa (m), Vectastain Abc-ap Mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Fluorescence Microscopy. Prostate: Psa (m), Vectastain Abc-ap Mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Fluorescence Microscopy. Vectorlabs
Melanoma Was Stained with Anti-vimentin Followed by Immpress™-ap Anti-rabbit Igg Reagent and Bcip/nbt Substrate. Note the Excellent Contrast of the Substrate with the Brown Pigments. Melanoma Was Stained with Anti-vimentin Followed by Immpress™-ap Anti-rabbit Igg Reagent and Bcip/nbt Substrate. Note the Excellent Contrast of the Substrate with the Brown Pigments. Vectorlabs
Prostate: Prostate Specific Antigen (m), Vectastain Abc-ap Kit, Bcip/nbt Substrate Kit (indigo). Prostate: Prostate Specific Antigen (m), Vectastain Abc-ap Kit, Bcip/nbt Substrate Kit (indigo). Vectorlabs
Colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy.colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy. Colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy. Vectorlabs
Colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Fluorescence Microscopy. Colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Fluorescence Microscopy. Vectorlabs
Bcip/nbt Substrate Kit, Alkaline Phosphatase (ap), (5-bromo-4-chloro-3-indolyl Phosphate/nitroblue Tetrazolium) Bcip/nbt Substrate Kit, Alkaline Phosphatase (ap), (5-bromo-4-chloro-3-indolyl Phosphate/nitroblue Tetrazolium) Vectorlabs
Bcip/nbt Substrate Kit, Alkaline Phosphatase (ap), (5-bromo-4-chloro-3-indolyl Phosphate/nitroblue Tetrazolium) Bcip/nbt Substrate Kit, Alkaline Phosphatase (ap), (5-bromo-4-chloro-3-indolyl Phosphate/nitroblue Tetrazolium) Vectorlabs
Prostate: Psa (m), Vectastain Abc-ap Mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy. Prostate: Psa (m), Vectastain Abc-ap Mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy. Vectorlabs
Prostate: Psa (m), Vectastain Abc-ap Mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Fluorescence Microscopy. Prostate: Psa (m), Vectastain Abc-ap Mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Fluorescence Microscopy. Vectorlabs
Melanoma Was Stained with Anti-vimentin Followed by Immpress™-ap Anti-rabbit Igg Reagent and Bcip/nbt Substrate. Note the Excellent Contrast of the Substrate with the Brown Pigments. Melanoma Was Stained with Anti-vimentin Followed by Immpress™-ap Anti-rabbit Igg Reagent and Bcip/nbt Substrate. Note the Excellent Contrast of the Substrate with the Brown Pigments. Vectorlabs
Prostate: Prostate Specific Antigen (m), Vectastain Abc-ap Kit, Bcip/nbt Substrate Kit (indigo). Prostate: Prostate Specific Antigen (m), Vectastain Abc-ap Kit, Bcip/nbt Substrate Kit (indigo). Vectorlabs
Colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy.colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy. Colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Bright Field Microscopy. Vectorlabs
Colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Fluorescence Microscopy. Colon: Ae1/ae3 (m), Immpress-ap Anti-mouse Igg Kit, Bcip/nbt (indigo), Viewed Under Fluorescence Microscopy. Vectorlabs
Bcip/nbt Substrate Kit, Alkaline Phosphatase (ap), (5-bromo-4-chloro-3-indolyl Phosphate/nitroblue Tetrazolium) Bcip/nbt Substrate Kit, Alkaline Phosphatase (ap), (5-bromo-4-chloro-3-indolyl Phosphate/nitroblue Tetrazolium) Vectorlabs
Bcip/nbt Substrate Kit, Alkaline Phosphatase (ap), (5-bromo-4-chloro-3-indolyl Phosphate/nitroblue Tetrazolium) Bcip/nbt Substrate Kit, Alkaline Phosphatase (ap), (5-bromo-4-chloro-3-indolyl Phosphate/nitroblue Tetrazolium) Vectorlabs
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Description

BCIP/NBT AP Substrate Kit forms an indigo, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.

Features:

  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation: 645-685 nm*
  • Emission: peaks at 823 and 855 nm*

*Biotechniques (2007) 42:756-759 and BioTechniques (2014) 57:254-256

Specifications

More Information
Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)Alkaline Phosphatase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorIndigo
Color of FluorescenceFar Red
MicroscopyBrightfield, Fluorescence, Spectral Imaging
Additional Substrate PropertiesContrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
MountingAqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Safety TitleWARNING: Reproductive Harm – www.P65Warnings.ca.gov

Kit Contents:

  • 4 ml BCIP/NBT Reagent 1
  • 3 ml BCIP/NBT Reagent 2
  • 4 ml BCIP/NBT Reagent 3

Documents

Citations

Technical Information

This substrate can be used for developing blots or for in situ hybridization. At 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. This kit contains stock solutions in convenient dropper bottles.

Substratesensitivity2 Jpg Substratesensitivity2 Vectorlabs  Substrate.counterstainchart Jpg Substrate.counterstainchart Vectorlabs

Substratecombos Jpg Substratecombos Vectorlabs

Applicable patents and legal notices are available at legal notices.

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