AZDye™ 594 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice in live cell surface glycans and lipids studies where the presence of cytotoxic copper catalyst is not acceptable.
AZDye™ 594 is bright, water-soluble, and pH-insensitive from pH 4 to pH 10 red-fluorescent dye with absorption and emission maxima at 590 and 617 nm, respectively. It can be used with the 561 nm and 594 nm laser lines. AZDye™ 594 dye structurally is identical to Alexa Fluor® 594. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including DyLight® 594, CF® 594 Dye, Texas Red®, and ATTO 594.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 590/617 nm |
| Extinction Coefficient | 88,000 |
| Spectrally Similar Dyes | Alexa Fluor® 594, CF® 594, DyLight® 594 |
| Molecular weight | 980.10 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Appearance | Dark blue/purple solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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