Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.

AZDye 568 Picolyl Azide (CCT-1292)

[text-backorder]

This product was previously sold as 1085 from Fluoroprobes.

Description

AZDye™ 568 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

AZDye 568™ is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AZDye™ 568 dye structurally is identical to Alexa Fluor® 568 Dye. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including CF® 568 Dye, Alexa Fluor® 568.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima578/602 nm
Extinction Coefficient
88,000
Flow Cytometry Laser Line
532 nm or 568 nm
Microscopy Laser Line
532 nm or 568 nm
Spectrally Similar DyesAlexa Fluor® 568, CF® 568
Molecular weight897.25
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF568

Selected References

  1. Ratnayeke, N., et al. (2022). CDC7-independent G1/S transition revealed by targeted protein degradation. Nature.605 (7909), 357-365. [PubMed]
  2. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
  3. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,25, 698-706. [PubMed]
  4. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,.51, 5852-56. [PubMed]

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Log in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote