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AZDye 555 Tyramide (CCT-1540)

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Description

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.

AZDye™ 555 Tyramide (Alexa Fluor® 555 Tyramide equivalent) is a component of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Specifications

Unit Size 150 slides, 1500 slides
Abs/Em Maxima 555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar Dyes Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight 966.16 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

Abs/Em Spectra

AF555

Applicable patents and legal notices are available at legal notices.

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