AZDye™ 532 Alkyne is a bright, yellow-fluorescent, alkyne-activated probe that reacts with azides via a copper-catalyzed click reaction (CuAAC). This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye 532 dyes are well suited to direct imaging of low-abundance targets.
The absorption/emission spectra of AZDye™ 532 is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.
For application where the presence of copper is not acceptable, please consider our AZDye™ 532 DBCO probe for copper-less detection of azide-modified molecules.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 532/554 nm |
| Extinction Coefficient | 78,000 |
| Flow Cytometry Laser Line | 532 nm |
| Microscopy Laser Line | 532 nm |
| Spectrally Similar Dyes | Alexa Fluor® 532, Atto™ 532, CF® 532 dyes |
| Molecular weight | 651.61 |
| Solubility | Water, DMSO, DMF |
| Appearance | Orange to light red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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