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AZDye 488 Picolyl Azide (CCT-1276)

[text-backorder]

This product was previously sold as 1019 from Fluoroprobes.

Description

AZDye™ 488 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima494/517 nm
Extinction Coefficient
73.000
Spectrally Similar DyesFluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488
Molecular weight736.69 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceYellow solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Selected References

  1. Ratnayeke, N., et al. (2022). CDC7-independent G1/S transition revealed by targeted protein degradation. Nature.605 (7909), 357-365. [PubMed]
  2. Ratnayeke, N., et al. (2021). Cdt1 inhibits CMG helicase in early S phase to separate origin licensing from DNA synthesis. bioRxive-print. [bioRxiv]
  3. Köberlin, M. S., et al. (2021). LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components. J Cell Biol.220 (8), e202009147. [PubMed]
  4. Morral, C., et al. (2020). Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro. STAR ProtocolsVolume 1, 2 [ScienceDirect]
  5. Uchiyama, J., et al. (2020). Quantitative nascent proteome profiling by dual pulse labeling with O-propargyl-puromycin and stable isotope labeled amino acids. The Journal of Biochemistry10, 1093. [Oxford Academic]
  6. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,25, 698-706. [PubMed]
  7. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,.51, 5852-56. [PubMed]
  8. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

Applicable patents and legal notices are available at legal notices.

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