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AZDye 488 DBCO (CCT-1278)

[text-backorder]

This product was previously sold as 1021 from Fluoroprobes.

Description

AZDye™ 488 DBCO is a water-soluble, green-fluorescent probe for copper-less detection of azide-tagged biomolecules. In application where the presence of copper is a concern AZDye™ 488 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, CF® 488 Dye and Alexa Fluor® 488.

DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademarks of Biotium Inc.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima494/517 nm
Extinction Coefficient
73,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
CF260
0.35
CF280
0.19
Spectrally Similar DyesFAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Molecular weight792.12 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceOrange to light red solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Selected References

  1. Loebel , C., et al. (2022). Metabolic labeling of secreted matrix to investigate cell-material interactions in tissue engineering and mechanobiology. Nat Protoc.10.1038, Online ahead of print. [PubMed]
  2. Lancien, M., et al. (2020). A snake toxin as a theranostic agent for the type 2 vasopressin receptor. Theranostics.10 (25), 11580-11594. [PubMed]
  3. Simon P. Wisnovsky, et al. (2020). Metabolic precision labeling enables selective probing of O-linked N-acetylgalactosamine glycosylation. PNAS, 117 (41), 25293-25301. [PNAS]
  4. Loebel, C., et al. (2020). Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte–Hydrogel Interface. Adv. Funct. Mater. [PubMed]
  5. Loebel, C., et al. (2019). Local nascent protein deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels. Nature Materials, 18, 883-891. [PubMed]

Applicable patents and legal notices are available at legal notices.

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