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AZDye 488 Alkyne (CCT-1277)

[text-backorder]

This product was previously sold as 1020 from Fluoroprobes.

Description

AZDye™ 488 Alkyne is a bright, green-fluorescent alkyne-activated probe routinely used for imaging of low abundance azide-containing biomolecules. AZDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. A probe for copper-less azide detection (AZDye™ 488 DBCO) is also available for application where the presence of copper is not acceptable.

AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets. AZDye™ 488 is structurally identical to Alexa Fluor® 488 Alkyne. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488 and CF® 488 Dye.

Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima494/517 nm
Extinction Coefficient
73,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar DyesFAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488
Molecular weight571.53 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceOrange to light red solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Selected References

  1. Tharp, J. M., et al. (2021). Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons. ACS Chem Biol.16 (4), 766-774. [PubMed]
  2. Bruna, R. E., et al. (2021). Limitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis. Proc Natl Acad Sci U S A.118 (11), e2021370118. [PubMed]
  3. Kwong, J. Q., et al. (2021). Mitochondrial functional resilience after TFAM ablation in the adult heart. Am J Physiol Cell Physiol.320 (6), C929-C942. [PubMed]
  4. Schuler, D., et al. (2021). Differential Labeling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis. Microorganisms9, 1578. [MDPI]

Applicable patents and legal notices are available at legal notices.

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