AQuora® PB NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® PB NHS Ester (Pacific Blue) yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® PB NHS Ester is a coumarin-based dye with photophysical properties comparable to Pacific Blue.
This AQuora® PB NHS Ester (Pacific Blue) dye is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.
Pacific Blue is a trademark of Thermo Fisher Scientific.
| Unit Size | 1mg |
|---|---|
| Format | Lyophilized |
| Storage | Store at -20°C. Protect from light. May ship at ambient temperatures. |
| Country of Origin | United States of America |
| Label | AQuora® PB |
| Excitation / Emission Maximum | 404 nm / 458 nm |
| A280 Correction Factor | 0.400 |
| Reactive Group | NHS Ester |
| Extinction Coefficient | 19,000 |
| Spectrally Similar Dyes | Pacific Blue |
| Molecular Weight | 940 |
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